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Biotechnology

Genetic Engineering. The main direction of research is the production of recombinant forms of enzymes and peptides. The laboratory carries out a full cycle of work in the field of genetic engineering: from the choice of a strategy for cloning an individual gene and its chemical-enzymatic synthesis to the development of a method for isolation, purification, complete physical and chemical identification and study of biological activity.

Most important results

Efficient technologies for obtaining recombinant drugs for pharmacology and the food industry have been developed. Pilot batches of defensin, an antimicrobial peptide from the seeds of Nigella sativa L., have been produced. Patent application No. IAP 2018 0267 has been filed.
E.coli C43-LDH and C43-GOX cell lines, stably expressing lactate dehydrogenase and glucose oxidase enzymes, were obtained
For the first time, the genes of lactate dehydrogenase B from the heart of a bull and glucose oxidase from Aspergillus Niger were cloned into vector systems pET45b. The resulting recombinant molecules were transformed into two types of E. coli cells — BL21 and C43.
The enzymes lactate dehydrogenase and glucose oxidase are ready for use in the production of domestic reagent kits for biochemical analysis.
For the first time in Uzbekistan, a domestic test system for detecting SARS-CoV-2 coronavirus RNA by real-time PCR was developed and a patent for utility model No. FAP 02010 was obtained.
The scientists of the laboratory performed sequencing of the S gene region and whole genome sequencing of the SARS-CoV-2 coronavirus genome. The results of the study were published in the international databases NCBI and GISAID, as well as in the authoritative journal PLOS ONE (impact factor 3.5).
Together with the Institute of Microbiology of the Chinese Academy of Sciences, Anhui Zhifei Longcom Biopharmaceutical (PRC) and the Biotechnology Laboratory of the Center for Advanced Technologies, the third stage of clinical trials of a recombinant vaccine against the SARS-CoV-2 coronavirus was conducted in 2020-2021. The research results were published in the authoritative journal The new england journal of medicine. Scientists from the Biotechnology Laboratory studied strains of coronavirus common in Uzbekistan and, based on the data obtained, two types (DNA and recombinant) of vaccines against SARS-CoV-2 coronavirus were developed. The testing laboratory of the Center of Excellence was accredited according to the ISO 17025 standard – Methods of analysis for the detection of genetically modified organisms and derived products
                                                                                             Medical genomics.

The main direction of the laboratory’s research lies in the field of human genetics and consists in understanding the genetic basis of hereditary (congenital pathologies) and multifactorial human diseases. Genetic polymorphisms of genes that lead to the risk of developing diseases, as well as genetic mutations responsible for the development of congenital pathologies, are being studied.
                                                                                          Most important result

Research is being actively carried out in the field of studying the genetics of many complex diseases. To date, as a result of the research, more than 150 genetic markers have been identified for 62 genes of predisposition to 28 hereditary diseases characteristic of people of Uzbek nationality.
A blood and DNA bank has been collected, a total of 6000 DNA samples (6000 total or only DNA) of all nationalities living in Uzbekistan. The basics of molecular genetic predisposition to essential hypertension have been studied.
More than 600 patients were studied for 15 genes (ACE, β-1 Adrenoreceptor, β-2 Adrenoreceptor, β-3 Adrenoreceptor, 11b-Hydroxysteroid Dehydrogenase Type 2, Type 2 Angiotensin II Receptor (AT2), Mineralocorticoid Receptor, IRS I, IRS II, G-protein β-3 subunit gene, Alpha-Adducin, Angiotensinogen, APOE, PPARγ2). The genetic component of bronchial asthma was studied, the significance and prevalence of polymorphisms of candidate genes of bronchial asthma, such as the CC16 gene, the ADRB2 gene, and the high-affinity FCER1B gene, were determined. As a result of the research work, an association of the null allele of the GSTM1 and GSTT1 genes, as well as the 38A/38A polymorphism of the CC16 gene with the formation of bronchial asthma, was revealed. It has been established that the genes of the xenobiotic detoxification system (GSTM1, GSTT1, GSTP1) and the CC16 gene are an important part of the genetic structure of susceptibility to bronchial asthma.
                                                                         The molecular genetic basis for the dev

elopment of migraine, coronary heart disease – ApoB, LDLR; blood clotting factors – FV, FII, MTHFR; type 2 diabetes – e-nos, ACE, IRS, TCF7L2; stroke – ITGA2, GP1BA, ITGB3, FGB; lactase intolerance–LCT polymorphism. The genetics of the human gastric pathogen Helicobacter pylori has been studied. The genetic heterogeneity of clinical isolates distributed on the territory of Uzbekistan was revealed. Genotyping by virulence genes was carried out and bacterial genotypes associated with stomach diseases were determined. The most aggressive strains of bacteria common in the republic were identified.glare. Whole-genome scanning of cultivated clinical isolates of H. pylori was carried out using the Microarray method (microchips), strain-specific genes were identified and their distribution by functional categories was analyzed. The VacA gene was sequenced in 30 selected isolates. Genetic hereditary pathologies The phenylalanine hydroxylase gene has been sequenced in patients with phenylketonuria, and for the first time in the world, previously undescribed mutations leading to the development of phenylketonuria have been identified. Methods have been developed for diagnosing genetic diseases of an endocrine nature: congenital adrenal hyperplasia, phenylketonuria, Duchenne myodystrophy, Shereshevsky-Turner syndrome, Klinefelter syndrome, adrenogenital syndrome. Techniques are effectively introduced into medical practice. For the first time, a technique has been developed to identify the origin of the parental chromosome in Shereshevsky-Turner syndrome. The molecular genetic bases of predisposition to various types of sports activities have been studied. For the first time in the Republic, a molecular genetic analysis of AMPD1, ACTN3, ACE, PPARA, PPARD, PPARG gene polymorphisms in athletes was carried out.